It is difficult to achieve high performance without sufficient training data for deep convolutional neural networks (DCNNs) to learn. Data augmentation plays an important role in improving robustness and preventing overfitting in machine learning for many applications such as image classification. In this paper, a novel method for data augmentation is proposed to solve the problem of machine learning with small training datasets. The proposed method can synthesise similar images with rich diversity from only a single original training sample to increase the number of training data by using generative adversarial networks (GANs). It is expected that the synthesised images possess class-informative features, which may be in the validation or testing data but not in the training data due to that the training dataset is small, and thus they can be effective as augmented training data to improve classification accuracy of DCNNs. The experimental results have demonstrated that the proposed method with a novel GAN framework for image training data augmentation can significantly enhance the classification performance of DCNNs for applications where original training data is limited.