A multitude of similar or even identical objects are positioned closely in dense scenes, which brings about difficulties in object-detecting and object-counting. Since the poor performance of Faster R-CNN, recent works prefer to detect dense objects with the utilization of multi-layer feature maps. Nevertheless, they require complex post-processing to minimize overlap between adjacent bounding boxes, which reduce their detection speed. However, we find that such a multilayer prediction is not necessary. It is observed that there exists a waste of ground-truth boxes during sampling, causing the lack of positive samples and the final failure of Faster R-CNN training. Motivated by this observation we propose a multiple-step sampling method for anchor sampling. Our method reduces the waste of ground-truth boxes in three steps according to different rules. Besides, we balance the positive and negative samples, and samples at different quality. Our method improves base detector (Faster R-CNN), the detection tests on SKU-110K and CARPK benchmarks indicate that our approach offers a good trade-off between accuracy and speed.